HPV – Corewell Health Laboratory

Advanced Technology Laboratory, Cytology, General Information

Reflex HPV Testing Update

Effective April 2, 2025, the Reflex HPV algorithm will more closely align with ASCCP recommendations. HPV testing will no longer be performed for a normal Pap result in patients aged 30 years and older when a provider orders HPV Reflex.

Specimens will qualify for HPV Reflex with a cytologic diagnosis of:

ASCUS (Atypical Squamous Cells of Undetermined Significance) for patients aged 21 and older.
LSIL (Low Grade Squamous Intraepithelial Lesion) for patients aged 25 and older.

HPV testing will continue to be performed for all Paps, regardless of results, for patients aged 30 years or older when a provider orders Co-Testing.

If a provider wishes to request HPV testing on a normal pap for patients under 30, they must either place an electronic order at the time of the initial pap order or request an add-on test for:

HPV High Risk Screen with HPV Genotype Reflex – Cervical Source (LAB1231770)
HPV High Risk Screen with HPV Genotype Reflex – Vaginal Source (LAB1230722)

Updated Pap and HPV Testing Algorithm

March 28, 2025 by Laboratory Services

Advanced Technology Laboratory

Human Papillomavirus (HPV) Testing Update

Corewell Health South and Corewell Health West Laboratories

Effective Date: February 3, 2025

Human Papillomavirus (HPV) Genotypic testing will be updated to expand acceptable sources and collection methods. The following HPV test will be available, and the corresponding testing algorithm will be driven by the clinician specified specimen source and collection method/device.

New Test: Self-Collect HPV High-Risk Screen with HPV Genotype Reflex (LAB1231853)

Testing Restricted to HPV only –Co-testing Not Available: PAP or STI (CT, NG, TV)
Detection of 14 HR HPV genotypes:
- HPV Genotype 16
- HPV Genotype 18
- Other HR HPV – includes 31,33,35,39,45,51,52,56,58,59,66,68
Collection Information:
- Acceptable Source: Patient Self-collected vaginal
- Collection Device: Rovers® Evalyn® Brush (ITM-1303902)
- Collection Instructions: Patients must use the Evalyn Brush in a healthcare setting (i.e. physician office or clinic) to self-collect a vaginal specimen following the Evalyn Brush collection instructions. (Spanish instructions)
- A trained healthcare staff member must immediately suspend the Evalyn Brush into a vial of ThinPrep PreservCyt solution following transport instructions. The ThinPrep PreservCyt vial can then be transported to the lab at 2 to 30°C.
- This may not be collected at home or in an outpatient draw site.

Corewell Health Southwest: Effective February 3, 2025, PAP and HPV results will once again be bundled for all orders placed from that date onward.

January 28, 2025 by Laboratory Services